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By I. R. Lehman, Janice R. Chien (auth.), Robert D. Wells, Ross B. Inman (eds.)

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1972. In preparation. 6. , and Kornberg, A. 1972. Enzymatic synthesis of deoxyribonucleic acid. J. Bioi. Chem. 247: 241. 7. Cook, J. S. 1972. In Beers, R. , and Herriot, R. M. ) Molecular and cellular repair processes, Johns Hopkins Press, Baltimore, Md. (In press). 8. Dulbecco, R 1950. Experiments on photoreactivation of bacteriophages inactivated with ultraviolet radiation. J. Bacteriol. 59: 329. 9. Friedberg, E. , and King, J. J. 1971. Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: Purification and characterization of a dimer-specific phage-induced endonuclease.

5 "tI " E L 0 ~ ~ a. 2 4 6 f 0 24 nours l: l- "tI Fig. 1. Synthesis and turnover of poly (dT) with poly (dA)n as template. M); DNA polymerase (large fragment) (33 nM). M (open symbols). The reaction mixture was preincubated at 22°C for 30 min before addition of the large fragment. 08 M ammonium formate for 16 hr. The origin and dTMP spots were cut out and counted in a liquid scintillation counter. 16 KLENOW AND OVERGAARD-HANSEN synthesis of polymer in excess of the amount of poly (dA) present. Any poly (dT) in excess of poly (dA) is, however, immediately degraded to dTMP by the 3' ~ 5' exonuclease activity.

Luteus. A further discussion of this phenomenon will be found in the section dealing with reinsertion. Excision Step Nucleotide and photoproduct removal in vitro is dependent on a second nuclease in the excision-repair cycle. This enzyme acts exonucleolytically on single stranded DNA, initiating hydrolysis from either the 5' or 3' terminus (12). Unlike most known exonucleases, the M. luteus exonuclease, which is capable of participating in excision in vitro, is largely unaffected in its rate and extent of hydrolysis by the presence of thymine dimers.

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