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By G. Fred & Sumner, G. Fred Somers

Illustrated / / Sciences / Engels / English / Anglais / Englisch / demanding conceal / 15 x 24 cm / 415 .pp /

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Of albumin and 10 mg. of calcium chloride. The total volume of digest is 13 cc. Amylase units are expressed as the monomolecular reaction constant. The enzyme acts upon 25 cc. of 1 per cent of soluble starch at 37°C. 8 is maintained by 10 cc. 2 N phosphate buffer. One adds 1 cc. 2 N sodium chloride to activate the (animal) amylase. The total volume is 37 cc. and the maltose produced is determined by hypoiodite titration (Willstätter, Waldschmidt-Leitz, and Hesse, 252). Catalase units can be expressed as the monomolecular reaction constant, extrapolated to zero time, for the decomposition of hydrogen peroxide under standard conditions.

26 CHEMISTRY AND METHODS OF ENZYMES Urease activity is expressed by choosing as the unit that amount of urease which will form 1 mg. of ammonia nitrogen from urea in 5 minutes at 20°C. 0. The reacting mixture contains 1 cc. 6 per cent neutral phosphate and 1 cc. of enzyme preparation. The reaction is stopped by adding 1 cc. of N hydrochloric acid (211). 5 g. of olive oil in 1 hour at 30°C. 9 and in the presence of 15 mg. of albumin and 10 mg. of calcium chloride. The total volume of digest is 13 cc.

Inactivation by Irradiation. General reference: (137). It has been known for many years that various enzymes are inactivated to different degrees by the action of radiations of various wavelengths. At wavelengths greater than about 3000 A the presence of photosensitizers is necessary to obtain inactivation (137). Collier and Wasteneys (48) found that urease, malt amylase, pepsin, and a phosphatase were inactivated by ultraviolet light of 200 to 313 ιημ, that urease was inactivated by infrared light of 750 to 1400 ιημ, that phosphatase was inactivated by visible light and that while amylase was not affected by visible light, it was inactivated in the presence of starch.

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