By J. Vandepitte, K. Engbaek, P. Piot, C.C. Heuck, P. Rohner
This guide is a pragmatic advisor, to be used by means of laboratory employees in healthiness centres and district hospitals, to the strategies to be in acquiring specimens, keeping apart and choosing micro organism, and assessing their resistance to antibiotics. It covers bacteriological research of blood, cerebrospinal fluid, urine, stool, sputum, pharyngeal and genital specimens, and purulent exudates. specific cognizance is given to the necessity for quality controls of all laboratory systems. a listing of media and reagents wanted for the isolation and id of the commonest bacterial pathogens is integrated, including a sign in their relative significance for the middleman laboratory. This checklist is meant for variation to neighborhood situations.
This moment variation has been up to date in lots of components, together with a tremendously improved part on stool specimens and a brand new part on serological assessments.
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Meningitis or encephalitis. Only the microbiological examination of CSF will be discussed here, although the CSF leukocyte count is also of paramount importance. The most common causal agents of meningitis are listed in Table 6 according to the age of the patient, but it should be kept in mind that some overlap exists. Collection and transportation of specimens Approximately 5–10 ml of CSF should be collected in two sterile tubes by lumbar or ventricular puncture performed by a physician. In view of the danger of iatrogenic bacterial meningitis, thorough disinfection of the skin is mandatory.
Deposit 1 ml of the urine on a blood agar plate and streak half the plate by making a straight line down the centre (1), followed by close passes at right angles through the original (2), and ending with oblique streaks crossing the two previous passes (3) (Fig. 3). 3. Inoculate the MacConkey agar in the same manner. 4. Incubate the plates overnight at 35 ∞C. g. CLED1 agar, purple lactose agar). Filter-paper dip-strip method The filter-paper dip-strip method of Leigh & Williams2 is based on the absorption and subsequent transfer of a fixed amount of urine to a suitable plating agar medium.
Rapid diagnosis of Enterobacteriaceae. 1. Detection of bacterial glycosidases. Acta Pathologica et Microbiologica Scandinavica, Section B, 1976, 84:245–251. 35 A URINE Procedure: 1. 25 ml of saline. The suspension should be prepared from colonies growing on MacConkey agar. 2. 5). 1. 25 ml of the medium into each of the required number of sterile tubes. Close the tubes with stoppers. Label the tubes PGUA and indicate the date. 3. Inoculate one tube of the PGUA medium with a dense suspension of the organism to be tested.