Download Aqueous Two-Phase Systems: Methods and Protocols (Methods in by Rajni Hatti-Kaul PDF

By Rajni Hatti-Kaul

A mix of polymers, or one polymer and a salt, in an aqueous medium separates into stages: this phenomenon turns out to be useful in biotechn- ogy for product separations. Separation of organic molecules and debris in those aqueous two-phase structures (ATPS) was once initiated over forty years in the past by means of P.-Å. Albertsson, and later proved to be of substantial software in biochemical and cellphone organic examine. a lift within the software of ATPS used to be noticeable while difficulties of separations in biotechnology strategies have been encountered. Its simplicity, biocompatibility, and amenability to effortless scaleup operations make using ATPS very beautiful for large-scale bioseparations. regardless of the benefits ATPS enjoys over different separation suggestions, the applying of two-phase structures has for a very long time been limited to chose labora- ries. fresh years have, notwithstanding, proven a development during which expanding numbers of researchers hire two-phase partitioning strategies in either easy and utilized examine.

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Extra resources for Aqueous Two-Phase Systems: Methods and Protocols (Methods in Biotechnology)

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7. 0 M KCl. 8. Concentrated ammonia. Items 3–8 are reagent grade. 3. Electrophoretic Mobility of Phase Drops Apparatus: A temperature controlled microelectrophoresis apparatus is required, such as the Mark I or Mark II from Rank Bros. (Bottisham, Cambridge, UK), in which the drop is observed directly or by video. The more 40 Brooks and Norris-Jones automated laser doppler instruments will not provide sufficiently accurate individual drop sizes. The instrument must be equipped with an eyepiece filar micrometer such as sold by American Optical (Buffalo, NY) or with a calibrated optical field analyzer for video images.

The chamber is illuminated with a Nikon fiber optic light source with a frosted glass diffuser between the fiber and the chamber. 0-mL tuberculin syringe whose lateral location is controlled with a micromanipulator. Instead of a needle, a very fine tip is drawn from a microcapillary over a suitable flame and cut to provide a small Physical Properties of ATPS 2. 3. 4. 5. 6. 7. 8. 008 cm) for small drops. 0 cm are cut to carry or act as the test surfaces. Software: A standard image-analysis package, such as OPTIMAS™ is used to obtain the drop profile.

A more precise method is by analysis of the top and bottom phase composition where the TLL = 6 ( X 2 + 6Y 2)1/2, X denotes the concentration of component 1 and Y, the concentration of component 2 (see Fig. 1). However, if the total composition and weight ratio are known, then analysis of one phase is sufficient by using, Vtpt/Vbpb = Xb – X0/X0 – Xt where X denotes the concentration of component 1 in the top phase (t), bottom phase (b) and the total system (0) (the same holds true for component 2) (1).

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