Download Aqueous two-phase systems. Methods and protocols by Rajni Hatti-Kaul PDF

By Rajni Hatti-Kaul

A mix of polymers, or one polymer and a salt, in an aqueous medium separates into levels: this phenomenon turns out to be useful in biotechn- ogy for product separations. Separation of organic molecules and debris in those aqueous two-phase platforms (ATPS) used to be initiated over forty years in the past by way of P.-Å. Albertsson, and later proved to be of significant application in biochemical and cellphone organic learn. a lift within the program of ATPS used to be obvious while difficulties of separations in biotechnology strategies have been encountered. Its simplicity, biocompatibility, and amenability to effortless scaleup operations make using ATPS very beautiful for large-scale bioseparations. regardless of the benefits ATPS enjoys over different separation strategies, the applying of two-phase structures has for a very long time been limited to chose labora- ries. fresh years have, even though, proven a development during which expanding numbers of researchers hire two-phase partitioning strategies in either simple and utilized learn.

Show description

Read or Download Aqueous two-phase systems. Methods and protocols PDF

Best diagnostics & labs books

Henry's Clinical Diagnosis and Management by Laboratory Methods, 21 e 2006

Required interpreting in medical pathology on the grounds that 1908! complete insurance offers an in-depth realizing of lab attempt choice and interpretation of effects. a brand new full-color structure makes reference a lot more uncomplicated. what is extra, new chapters on laboratory laws, foodstuff, point-of-care checking out, bioterrorism, proteomics, microarray research, and the human genome venture - in addition to a totally new part on melanoma checking out and meticulous updates from conceal to hide - positioned all of the most modern, such a lot crucial wisdom at your fingertips.

Examination of the hand and wrist

Content material: 1. useful Anatomy. Skeleton of the hand. Skeleton of the wrist. pursuits of the hand and wrist. epidermis disguise. services of the hand -- 2. medical exam of the Teguments, the Skeleton and the Musculotendinous gear. exam of the teguments, palmar aponeurotic lesions and trophicity.

Extra info for Aqueous two-phase systems. Methods and protocols

Example text

2. A drop is selected and the diameter either measured with an eye piece micrometer or the drop image captured if a video microscope is being used. Physical Properties of ATPS 43 3. The field is turned on and the time taken for the drop to move across a convenient number of divisions in the eyepiece determined (see Note 9). 4. The field is then reversed in direction and the measurement repeated with the drop moving in the opposite direction, if it is still in view vertically. The average of the inverse of the times recorded for a given drop should be used to calculate the mobility of the drop, not the average of the times themselves, as it is the velocity that is being averaged (see Note 10).

The solution is repeatedly taken above and below the cloud point; the binodal lies between these two points (shown by the “zig zag” line). 2. illustrates turbidometric titration where a series of systems (᭹) are prepared and titrated until a one-phase system is formed—the binodal lies just above this point (᭡). 3. illustrates the determination of nodes (᭿) for systems, which is accomplished by preparing a series of systems lying on different tie-lines (------᭹------) and analyzing the concentration of components in the top and bottom phase.

Most dextran/PEG systems will equilibrate in 30 min but drops can be left overnight to ensure complete equilibration, providing the temperature remains constant. 1 µL to ~1 µL, depending on the phase density difference, interfacial tension, viscosity and microcapillary syringe tip dimension. Larger drops deform too much to be analyzed accurately because of the low interfacial tensions. Drops that are essentially flat on top cannot be analyzed. A convenient magnification standard consists of 18- and 30-gauge injection needles.

Download PDF sample

Rated 4.43 of 5 – based on 46 votes